Department of Microbiology
Background:

The Department of Microbiology at Dr. Rajendra Prasad Central Agricultural University (RPCAU), Pusa, was established in 1981 as the first department in Bihar to offer advanced teaching in microbiology backed by strong research and development. Housed within the College of Basic Sciences & Humanities, the department has built recognised strengths in microbial diversity and biofertilization, as well as mushroom biology and technology (now under DoR), supported over the years by competitive grants from national agencies such as DBT, ICAR, and NHB. It maintains a diverse microbial germplasm viz. cyanobacteria, Azospirillum/Azotobacter, Bacillus and many more PGPB and beneficial fungi. A notable achievement has been the development and large-scale validation of a microbial-consortium-based bionutrient package for rice, which has improved soil fertility, reduced chemical fertilizer dependence, and enhanced grain yields in farmers’ fields. Recent research initiatives include projects on soil biodiversity and biofertilizer development, cyanobacterial inoculants for nutrient cycling, and studies on agromicrobe–pesticide interactions to ensure safer intensification of agriculture.
In addition to these contributions, the department is actively engaged in developing innovative microbial formulations, including rice bran beads and banana sap-based bio-formulations, both of which are currently under patenting process. It is also pioneering work on nano-based biofertilizers, aimed at improving bioaugmentation of micronutrients, delivery efficiency, and sustainability in modern farming systems. Alongside its research, the department plays a vital academic role by offering postgraduate programs (M.Sc. and Ph.D.) in microbiology and making significant contributions to undergraduate teaching. It actively supports the Student READY programme (Rural Entrepreneurship Awareness Development Yojana) as part of the B.Sc. and B.Tech. curriculum, providing students with practical exposure to microbial technology, field applications, and agri-enterprise opportunities. Through its integration of quality teaching, innovative research, and farmer-oriented outreach, the Department of Microbiology continues to serve as a hub of excellence, contributing to both scientific advancement and sustainable agricultural development in eastern India.

Research
Externally funded Project
Sr. No | Project Title | Funding Agency | Duration |
1. | AINP-Soil Biodiversity and Biofertilizer | ICAR | 2006 (continuing…) |
University-funded project
Ongoing
Sr. No | Project Title | Funding Agency | Duration |
1. | Combined impacts of zinc solubilizing bacteria and zinc oxide nanoparticles on biofortification of rice crop | RPCAU | 2024 to 2027 |
2. | Exploration of Microbial Diversity from Various Ecological Niches for enhanced degradation of organic waste | RPCAU | 2024 to 2027 |
Completed
Sr. No | Project Title | Funding Agency | Duration |
1. | Formulation and evaluation of liquid inoculants on pulse productivity and soil health | RPCAU | 22/ 02/ 2017 to 22/02/2020 |
2. | Formulation of inexpensive media for mass production of microbial inoculants to improve soil health and pulse productivity | RPCAU | 16.12. 2021 to 16.12.2023
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Important Publications
- Kumar Navnit, Kumari Geeta, Kumar Anil and Paswan Sudhir (2016) Influence of manuring and irrigation scheduling on system productivity, resource use efficiency, nutrient uptake and incidence of early shoot borer (Chilo infuscatellus) in sugarcane (Saccharum spp. Hybrid complex) and garlic (Allium sativum) intercropping system. Indian J Agron.61(3): 297- 306. Impact Factor – 0.08,Citation – DOI:https://doi.org/10.59797/ija.v61i3.4389
- Navnit Kumar, Geeta Kumari and Vipin Kumar (2017) Enhancing Crop Productivity and Soil Health from Intercropping Coriander with Autumn Sugarcane through Scheduling of Fertilizer and Irrigation. Indian Society of Soil Science 65, No. 3 (2017) DOI: 10.5958/0974-0228.2017.00034.2
- Geeta Kumari, S.K.Thakur, Vipin Kumar, Navnit Kumar and S.K.Singh (2019) Long term effect of fertilizer, farm-yard manure and lime on yield sustainability and soil organic carbon pools under soybean (Glysine-max) – wheat (triticum aestivum) cropping system in Alfisol. Journal of Soil and water conservation 18(2): 196-204. ISSN: 022-457x DOI: 10.5958/2455-7145.2019.00011.0 NAAS: 5.02
- Niharika Bharti, Geeta Kumari, Devendra Singh, Manindra Nath Jha, Navnit Kumar and sunita Kumari (2019) Prospecting inexpensive media for mass production of microbial inoculants at farmers field. International Journal of Chemical Studies Vol 7(6): 1435-1442 NAAS: 5.38 DOI:https://dx.doi.org/10.22271/chemi
- Geeta Kumari, Deepak Kumar, Devendra Singh, and Navnit Kumar (2020), Characterization of Microbial Inoculants and its Different Formulation For Mass Production. International Journal of Current Microbiology and Applied SciencesVol 9 (7): 621 – 627. https://doi.org/10.20546/ijcmas.2020.907.070
- Geeta Kumari, Sunita Kumari, K. K. Singh, Aman Jaiswal and Anshuman Kohli (2022) Soil health management of an Alfisol with INM in direct seeded rice-chickpea system, Journal of Soil and water conservation 21(4): 424-430, DOI:5958/2455-7145.2022.00054.6_
- Jaiswal, A., Kumari, G., Upadhyay, V. K., Pradhan, J., Singh, H., & Pramanik, K. (2023). A methodology of isolation for development of PGPR consortia. Pharma Innovation, 12(11), 803-809. thepharmajournal.com
- Upadhayay, V. K., Singh, A. V., Khan, , Jaiswal, A., Bharti Kukreti, and G., Kumari. (2024). Assessment of zinc-solubilizing bacterial isolates for plant growth promoting traits and water stress tolerance behaviour. International Journal of Advanced Biochemistry Research, 8(2),378-382. DOI: https://doi.org/10.33545/26174693.2024.v8.i2e.570
- Jaiswal, A., Kumari, G., Upadhyay, V. K., Pradhan, J., & Pramanik, H. S. K. (2023). A methodology to develop liquid formulation of biofertilizer technology. Pharma Innovation, 12(81), 875-881. thepharmajournal.com
- Solanki K, Pradhan J*, Singh H, Satpathy B, Jaiswal A, Kumari, G. (2025). Leveraging AI to Propel Growth in Regenerative Agriculture: From Fields to the Cloud. Agriculture Association of Textile Chemical and Critical Reviews Journal. 13(1):337-344 https://aatcc.peerjournals.net/
- Pradhan, J., Pramanik, K., Jaiswal, A., Kumari, G., Prasad, K., & Jena, C. (2024). Biosynthesis of secondary metabolites in aromatic and medicinal plants in response to abiotic stresses: A review. Journal of Experimental Biology and Agricultural Sciences, 12(3), 318–334. https:// jebas.org
- Geeta Kumari, Aman Jaiswal, Devashish Pathak, Himani Priya, Nandeesha CV, Jyostnarani Pradhan and Ram Lakhan Limba (2025) Manuscript “Development of Effective Microbial Consortia based liquid formulations for Secondary Treatment of Wastewater” Agriculture Association of Textile Chemical and Critical Reviews Journal. 13(2), 42-52 https://aatcc.peerjournals.net/
- Geeta Kumari, Aman Jaiswal, Jyostnarani Pradhan,Hemlata, Kiran, Samala Manoj Kumar,Devashish Pathak, and Nidhi (2025) Manuscript “Abiotic and biotic stress mitigation in rice using rhozospheric isolates for zinc solubilization” Agriculture Association of Textile Chemical and Critical Reviews Journal. 13(3), 229-235 https://aatcc.peerjournals.net/
- Kiran, Savita Kumari, Shipra Kumari, Seema Kumari, Geeta Kumari, Nidhi and Aman Jaishwal (2025) Optimizing bioactive compound extraction from Clitoria ternatea L. blue flowers: A comparative solvent study, Annals of Phytomedicine: An International Journal 14(1): 1-12, 2025 DOI: http://dx.doi.org/10.54085/ap.2025.14.1.68
- Navnit Kumar, Geeta Kumari and U.P.Singh (2019) Phosphorus and Sulphur Nutrition for Sugarcane. Published as a book in AkiNik Publications, New Delhi. ISBN: 9789389680034
- Geeta Kumari, Navnit Kumar, and B. Mishra (2020) Integrated nutrient Management for yield sustainability in acidic soils. Integrated Publications, New Delhi. ISBN: 978819478094
- Aman Jaiswal, Geeta Kumari, Himani Priya and Ms. Akhila Pole (2022) Effect of Designed Microbial Consortia for Treatment of Waste water. Vidya Kutir Publications, New Delhi. ISBN: 978-93-92403-46-0
- Geeta Kumari, Aman Jaiswal, Viabhav Kr. Upadhayay and Jyostnarani Pradhan (2023) published a book entitled” Techniques in Soil Microbiology” in Agro academics. ISBN: 978-93-943809-0-5.
- Kumar, A., Kumar, N., Kumari, Geeta., Rana, L. and Sahoo, S.K. (2022) Agriculture and climate change impacts and solutions. Published by Brillion Publishing, New Delhi – 110005, India. pp – ISBN: 978-93-92725-46-3.
- Geeta Kumari, Aman Jaiswal, Viabhav Kr. Upadhayay and Jyostnarani Pradhan (2023) published an edited book entitled” Microbes and regenerative agriculture” in Agrobios Reaearch. ISBN: 978-93-94380-00000.
- Jyostnarani Pradhan, Hemlata Singh, Aman Jaiswal, Geeta Kumari and Rajeev Kumar (2023) published an edited book entitled” Challenges in the Green World: Abiotic Stress and Plants” in Elite Publishing House. ISBN:978-93-5899-851-1.
- Kumar, Ajeet, Choudhary, C.S., Paswan, D., Kumar, B. and Arun, A. (2014) Sustainable Way for Enhancing Phosphorus Efficiency in Agricultural Soils through Phosphate Solubilizing Microbes – A An Asian Journal of Soil Science (An International Refereed Research Journal). 9 (2): 300-310 https://hal.science/hal-04676707.
- Sinha, K., Kumar, Ajeet., Kumari, A and Singh. A.K. (2024) The Integrated Effect of Organic Manure, Biofertilizer and Inorganic Fertilizer on Soil Properties, Yield and Quality in Sugarcane Plant-ratoon System under Calcareous Soil of Indo Gangetic Plains of India. Journal of Scientific Research and Reports. 30(5): 193-206. https://doi.org/10.9734/jsrr/2024/v30i51934.;https://hal.science/hal-04507744.
- Kumar, Ajeet., Singh, S.K., Meena, K., Sinha, S.K. and Rana L (2024) Groundwater contamination with nitrate and human health risk assessment of North East alluvial plains of Bihar. International Journal of Environment and Climate Change, 14(3), 17–31. https://doi.org/10.9734/ijecc/2024/v14i34016; https://hal.science/hal-04484961
- Kumar, Ajeet., Chattopadhyay, and Meena, S.K. (2023) Soil Fertility Assessment of Sugarcane Growing Villages in Samastipur District of Bihar. Environment and Ecology. 41 (2): 759-764, April-June 2023. https://hal.science/hal-04676939.
- Kumar, Ajeet., Meena, S.K., Singh, S.K., Sinha, S.K. and Singh, A.K. (2024) Carbon sequestration in sugarcane plant – soil system as influenced by nutrient integration practices under Indo-Gangetic plains of India. Journal of Advances in Biology & Biotechnology (JABB). 27(5), 116–125. https://doi.org/10.9734/jabb/2024/v27i5769.
- Kumar, Ajeet (2015) Spatial Distribution of Macro and Micro Nutrients in soils of Saharsa district of Bihar. An Asian Journal of Soil Science (An International Refereed Research Journal). 10 (2): 276-282. .https://hal.science/hal-04676717.
- Kumar, B., Sinha, S.K., Kumar, Ajeet., and Kumari, A. (2024). Exploring the Impact of Organic-Inorganic Coupling on Nutrient Use Efficiency and Cane Yield in Calcareous Soils of the Indo-Gangetic Plains of India. Journal of Advances in Biology & Biotechnology, 27(6), 644-656. https://doi.org/10.9734/jabb/2024/v27i6924; https://hal.science/hal-04588263.
- Kumar, , Meena, S.K., Sinha, S.K., Singh, A.K., Minnatullah and Singh, S. K. (2024) Isolation and biochemical characterization of endophytic bacterium Gluconacetobacter diazotrophocus from native sugarcane cultivar of middle gangetic plains of India. Indian Journal of ecology, 51(1): 104-112. DOI: https://doi.org/10.55362/IJE/2024/4202.
- Paswan, D., Arun, A., Kumar, Ajeet and Choudhary, C.S. (2014) Effect of NPK uptake at different growth stages of wheat (Triticum aestivum) for yield maximization. An Asian Journal of Soil Science (An International Refereed Research Journal). 9 (2): 265-270. https://hal.science/hal-04676702.
- De, N., Kumar, Ajeet., Singh, R. K., Rai, A. K. and Rai, M. (2006). Effect of biofertilizers on quality of vegetable pea (Pisum sativum). Plant archive. 6 (2): 525-527. https://www.cabidigitallibrary.org/doi/full/10.5555/20073052236
- Khan, A., Singh, A. V., Kukreti, B., Pandey, D. T., Upadhayay, V. K., Kumar, R., & Goel, R. (2024). Deciphering the impact of cold-adapted bioinoculants on rhizosphere dynamics, biofortification, and yield of kidney bean across varied altitudinal zones. Science of The Total Environment, 927, 172204. https://doi.org/10.1016/j.scitotenv.2024.172204
- Jaiswal, A., Koli, D. K., Pabbi, S., Priya, H., Kumar, A., Mishra, R., & Dhaked, B. S. (2022). Effect of protective polymers and storage temperatures on shelf life of cyanobacterial liquid formulation. Indian Journal of Ecology, 49(4), 1517-1525. http://dx.doi.org/10.55362/IJE/2022/3692
- Dhaked, B. S., Koli, G. K., Triveni, S., Reddy, R. S., Jaiswal, A., & Koli, D. K. (2022). Screening of potassium and zinc solubilizing bacteria for plant growth promoting properties (PGPR) from different rhizospheric soil. Pharma Innov. J, 11, 1315-1319. https://www.thepharmajournal.com/archives/2022/vol11issue2/PartS/11-2-80-392.pdf
- Kumari, G., Kumari, S., Singh, K. K., Jaiswal, A., & Kohli, A. (2022). Soil health management of an Alfisol with INM in direct seeded rice-Chickpea system. Journal of Soil and Water Conservation, 21(4), 424-430. DOI: 10.5958/2455-7145.2022.00054.6
- Meena, K. R., Satyam, S., Singh, A., Jaiswal, A., & Rai, D. (2022). Benchmarking of different microbes for their biosurfactants antifungal action against plant pathogens. Indian Journal of Experimental Biology (IJEB), 60(12), 931-938. https://doi.org/10.56042/ijeb.v60i12.58955
- Upadhayay, V. K., Chitara, M. K., Mishra, D., Jha, M. N., Jaiswal, A., Kumari, G.,& Sharma, A. (2023). Synergistic impact of nanomaterials and plant probiotics in agriculture: A tale of two-way strategy for long-term sustainability. Frontiers in Microbiology, 14, 1133968. https://doi.org/10.3389/fmicb.2023.1133968
- Nandeesha, C. V., Akbari, L. F., Jaiswal, A., Harsha, B. R., Patil, B., Bhaliya, C. M., & Jain, S. (2023). Control efficacy and yield response of different fungicides evaluated against anthracnose of green gram. Crop Protection, 174, 106432. https://doi.org/10.1016/j.cropro.2023.106432
- Kumari, G., Jaiswal, A., Upadhyay, V. K., Pradhan, J., Singh, H., & Pramanik, K. (2023). A methodology to formulate liquid-based Rhizobium inoculants by using different additives.
- Jaiswal, A., Kumari, G., Upadhyay, V. K., Pradhan, J., Singh, H., & Pramanik, K. (2023). A methodology to develop liquid formulation of biofertilizer technology. Pharma Innovation, 12(11), 875–881.
- Jaiswal, A., Kumari, G., Upadhyay, V. K., Pradhan, J., Singh, H., & Pramanik, K. (2023). A methodology of isolation for development of PGPR consortia. Pharma Innovation, 12(11), 803–809.
- Jaiswal, A., Kumari, G., Priya, H. & Pole,A (2022). Effect of designed microbial consortia for treatment of waste water. Vidya Kutir Publications. ISBN: 978-93-92403-46-0
- Kumari, G., Jaiswal, A., Pradhan, J. & Upadhayay, V (2023). Techniques in Soil Microbiology.AGROACADEMICS An Imprint of Agrobios (India). ISBN: 978-93-943809-0-5
- Pradhan, J., Singh, H., Jaiswal, A., Kumari, G., & Kumar, R. (2023). Challenges in the green world: Abiotic stress and plants. Elite Publishing House. https://elitepublishing.in/challenges-in-the-green-world-abiotic-stress-and-plants/
- Kumari, G., Jaiswal, A., Pradhan, J. & Upadhayay, V (2024). Microbes and regenerative agriculture. AGROBIOS RESEARCH An Imprint of Agrobios (India) Agro House. ISBN: 978-93-94380-00000
- Kumar, A., Jha, M. N., Singh, D., Pathak, D., & Rajawat, M. V. S. (2021). Prospecting catabolic diversity of microbial strains for developing microbial consortia and their synergistic effect on Lentil (Lens esculenta) growth, yield and iron biofortification. Archives of Microbiology, 203(8), 4913-4928.
- Pathak, D., Singh, D., Jha, M. N., & Choudhary, K. B. (2024). Harnessing the efficacy of a rhizobium strain in individual and consortium mode to promote sustainable lentil production and biofortification under diverse soil conditions. Journal of Soil Science and Plant Nutrition, 24(3), 5175-5194.
- Singh, M., Jha, S., Pathak, D., & Maisnam, G. (2025). Advancing biofertilizers: the evolution from single-strain formulations to synthetic microbial communities (SynCom) for sustainable agriculture. Discover Plants, 2(1), 226.
- Pathak, D., Sharma, P., Govindasamy, V. and Suman, A. (2025). Protocol for Quantitative Estimation of Hydrogen Cyanide Production from Bacteria. Bio-protocol15(18): e5441. DOI: 21769/BioProtoc.5441.
- Upadhayay, V. K., Singh, A. V., Khan, A., & Sharma, A. (2022). Contemplating the role of zinc-solubilizing bacteria in crop biofortification: An approach for sustainable bioeconomy. Frontiers in Agronomy, 4. https://doi.org/10.3389/fagro.2022.903321
- Upadhayay, V. K., Chitara, M. K., Mishra, D., Jha, M. N., Jaiswal, A., Kumari, G., Ghosh, S., Patel, V. K., Naitam, M. G., Singh, A. K., Pareek, N., Taj, G., Maithani, D., Kumar, A., Dasila, H., & Sharma, A. (2023). Synergistic impact of nanomaterials and plant probiotics in agriculture: A tale of two-way strategy for long-term sustainability. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1133968
- Adeleke, B. S., Chaudhary, P., Ayilara, M. S., Ojo, F. M., Erinoso, S. M., Upadhayay, V. K., Adeyemo, A. I., & Akinola, S. A. (2024). Rhizosphere Microbiomes Mediating Abiotic Stress Mitigation for Improved Plant Nutrition. Ecologies, 5(3), 375–401. https://doi.org/10.3390/ecologies5030024
- Mishra, D., Chitara, M. K., Upadhayay, V. K., Singh, J. P., & Chaturvedi, P. (2023). Plant growth promoting potential of urea doped calcium phosphate nanoparticles in finger millet (Eleusine coracana (L.) Gaertn.) under drought stress. Frontiers in Plant Science, 14. https://doi.org/10.3389/fpls.2023.1137002
- Upadhayay, V. K., Naik, Y. D., Ranjan, N., Kastury, C., Shekhar, S., Kumar, S., & Jaggi, V. (2023). Prolific Microbial Agents as Key Products for Sustainable Agriculture. In: Soni, R., Suyal, D.C., Morales-Oyervides, L. (eds) Microbial Bioactive Compounds. Springer, Cham. https://doi.org/10.1007/978-3-031-40082-7_10
- Upadhayay, V. K., de los Santos Villalobos, S., Aravindharajan, S. T. M., Kukreti, B., Chitara, M. K., Jaggi, V., Sharma, A., & Singh, A. V. (2024). Microbial Advancement in Agriculture. In: Chaudhary, P., Chaudhary, A. (eds) Microbial Inoculants. Interdisciplinary Biotechnological Advances. Springer, Singapore. https://doi.org/10.1007/978-981-97-0633-4_5
- Upadhayay, V. K., Maithani, D., Dasila, H., Taj, G., & Singh, A. V. (2023). Microbial services for mitigation of biotic and abiotic stresses in plants. In Advanced microbial techniques in agriculture, environment, and health management (pp. 67-81). Academic Press. https://doi.org/10.1016/B978-0-323-91643-1.00003-X.
Extension Activities
Domestication of mushroom production technology
Domesticated the mushroom production technology through training capsule on mushroom cultivation and spawn production through demonstration and display in Kisan mela, Krishi ghosti, villager’s site, mass media etc. Established a network of mushroom growers for proper technical advice, marketing and organized mushroom farming. Learning materials on mushroom production technology has also been developed and distributed to the users community. Counceling to the farmers on cyanobacterial biofertilizer and microbial consortium is also being provided.
Creation of entrepreneurs
Developed a human resources pool of 1693 on mushroom cultivation and 78 on mushroom spawn production, covering entire state. Technical guidance to trained personal resulted establishment of thirty one spawn production units in different districts of Bihar. Many poor families of Ranitola village of Samastipur district adopted mushroom as their source of livelihood. Translated the technology in each district of Bihar and many small entrepreneurs on mushroom production emerged in recent years due to our effort but still not in organized manner.
Microbial Expo
A microbial expo was organized during the year 2013 by the student of B.Tech (Biotechnology) in the department of Microbiology . Honorable Vice Chancellor Dr. R. K. Mittal inaugurated the event. Students displayed the microbial product viz. Neera: a health Drink; Azospirillum biofertilizer, Potato bread, Bacillus thuringensis based pesticide, Rhizobium biofertilizer, Cyanobacteria biofertilizer, Vinegar, Biochemical agents for pimples etc. developed by them under industrial microbiology programme. Vice chancellor appreciated the effort and suggested them to convert their innovative thought into commercial venture.
Faculty:
S.No. | Name | Position | Specialization | Contact No. | E-mail Id |
1. | Dr. Geeta kumari | Assistant Professor | Soil Biology | 9801332824 | geetasri.kumari5@gmail.com |
2. | Dr. Ajeet Kumar | Assistant Professor | Soil Biology | 9430571920 | ajeet.sri@rpcau.ac.in |
3. | Dr. Aman Jaiswal | Assistant Professor | Applied Microbiology | 7982654782 | aman.micro@rpcau.ac.in |
4. | Dr. Viabhav Kr. Upadhyay | Assistant Professor | Microbiology | 7982654782 | viabhav98@rpcau.ac.in |
5. | Dr. Devashish Pathak | Assistant Professor | Plant-Microbe Interaction | 8210692601 | devashishpathak104@rpcau.ac.in |
Infrastructure:
Central Instrumental Facility
| Culture Collection Room | Biocontrol and Molecular Lab |
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Food and Industrial Lab | PG Lecture Hall |
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